Saturday, December 29, 2012

PRRSv in wild boar populations in Korea


Abstract

No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU, type 1) or North American (NA, type 2) viruses, respectively. Two amplicons (one from type 1 and one from type 2) were used to analyze the PRRSV open reading frame 7 (ORF7) sequence. The nucleotide sequences of type 1 PRRSV ORF7 had identities between 96.1% and 98.4% with PRRSVs from domestic pigs in Korea. The sequences of type 2 PRRSV ORF7 had identities of 100% with the PRRSV strain VR-2332, which was prototypic North American strain. These results show that PRRSVs are present in wild boars in Korea, and effective PRRSV surveillance of the wild boar population might therefore be useful for disease control.
PMID:
 
23271179
 
[PubMed - in process]

Choi E, Lee C, Hyun B, Kim J, Lim S, Song J, Shin Y. A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea. Journal of veterinary science 2012;13(4):377-383.



Thursday, December 27, 2012

Immunopathogenesis of PRRSv in the respiratory tract


Abstract

Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) impairs local pulmonary immune responses by damaging the mucociliary transport system, impairing the function of porcine alveolar macrophages andinducing apoptosis of immune cells. An imbalance between pro- and anti-inflammatory cytokines, including tumour necrosis factor-α and interleukin-10, in PRRS may impair the immune response of the lung. Pulmonary macrophage subpopulations have a range of susceptibilities to different PRRSV strains and different capacities to express cytokines. Infection with PRRSV decreases the bactericidal activity of macrophages, which increases susceptibility to secondary bacterial infections. PRRSV infection is associated with an increase in concentrations of haptoglobin, which may interact with the virus receptor (CD163) and induce the synthesis of anti-inflammatory mediators. The balance between pro- and anti-inflammatory cytokines modulates the expression of CD163, which may affect the pathogenicity and replication of the virus in different tissues. With the emergence of highly pathogenic PRRSV, there is a need for more information on the immunopathogenesis of different strains of PRRS, particularly to develop more effective vaccines.
Copyright © 2012 Elsevier Ltd. All rights reserved.

Gomez Laguna J, Salguero F J, PallarÃs F J, Carrasco L. Immunopathogenesis of porcine reproductive and respiratory syndrome in the respiratory tract of pigs. The veterinary journal 2012 [in press].

Wednesday, December 12, 2012

Comparison of PCR assays for PRRSv detection using different sample types (semen, oral fluids, serum, blood swabs)

Abstract

The aims of this study were to compare three commercial porcine reproductive and respiratory syndrome virus (PRRSV) real-time RT-PCR assays for detection of genetically diverse PRRSV isolates in serum, semen, blood swabs, and oral fluids collected from experimentally-infected boars, and to evaluate the effects of sample pooling. Six groups of 3 boars negative for PRRSV were each inoculated with one of six PRRSV isolates (sharing 55-99% nucleotide sequence identity in ORF5). Samples were collected on days post-inoculation (dpi) -2, 1, 3, 5, 7, 14 and 21 and tested by one of three commercially available real-time RT-PCR assays (AB, TC, AD). At dpi 1, all assays detected at least one positive sample in each group. The highest detection rates were on dpi 3 and dpi 5. Between dpi 1 and 7, serum samples had the highest detection rate (90%) with 100% agreement between tests, followed by blood swabs (Kappa = 0.97) and semen (Kappa = 0.80). Oral fluids had the lowest detection rates (AB: 55%; TC: 41%; AD: 46%) and the highest disagreement between kits (Kappa = 0.63). Pools of five samples did not reduce the detection rates if there was one positive sample with a high amount of viral RNA in the pool. Serum and blood swab samples had shorter turn-around times for RNA extraction. The AB assay had a 1.6 times shorter PCR reaction time. In summary, serum and blood swabs had the best performance with highest detection rates and agreement between assays and shortest turn-around time.


PMID:
 
23224085
 
[PubMed - as supplied by publisher]




Gerber PF, O'Neill K, Owolodun O, Wang C, Harmon K, Zhang J, Halbur PG, Zhou L, Meng XJ, Opriessnig T. Comparison of commercial real-time RT-PCR assays for reliable, early and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected or negative boars using different sample types (semen, oral fluids, serum, blood swabs). 2012. Journal of clinical microbiology [accepted].





Thursday, December 6, 2012

Reproductive parameters following a PRRS outbreak where a whole-herd PRRS MLV vaccination strategy was instituted post-outbreak


Abstract

This study assessed the effect of whole-herd porcine reproductive and respiratory syndrome (PRRS) modified-live virus (MLV) vaccination on herd-level reproductive performance, PRRS virus (PRRSV) viremia, and antibody in a subset of females in a 1,200-sow commercial herd in Thailand. Following a PRRSV outbreak, the entire herd was vaccinated with PRRS MLV twice at 3-week intervals and at 3-month intervals, thereafter. Reproductive performance data over a 3-year period were available for analysis. Serum samples were collected before and after vaccination and tested by PRRSV ELISA and reverse transcription-polymerase chain reaction. Vaccination was statistically associated with a lower abortion rate (1.4 vs. 1.6 %), farrowing rate (83.8 vs. 90.0 %), total born (10.6 vs. 11.4 piglets/litter), liveborn (10.0 vs. 10.3 piglets/litter), stillbirths (4.6 vs. 7.0 %), mummies (0.7 vs. 1.6 %), and a higher return rate (11.3 vs. 5.9 %) when compared with the period before the PRRSV outbreak. Pregnant females vaccinated during early gestation farrowed fewer liveborn and more mummies than the comparison group, whereas females vaccinated during late gestation had a lower farrowing rate. In this herd, PRRS whole-herd vaccination had neutral, positive, and negative effects on reproductive performance. Thus, the decision to implement whole-herd vaccination should be balanced between the benefits derived from reproductive performance improvements, e.g., fewer abortions, stillborn piglets, and mummified fetuses, and the effect of vaccination on pregnant females.

Olanratmanee E, Nuntawan Na Ayudhya S, Thanawongnuwech R, Kunavongkrit A, Tummaruk P. Reproductive parameters following a PRRS outbreak where a whole-herd PRRS MLV vaccination strategy was instituted post-outbreak. Tropical animal health and production 2012 [in press].