Title: Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance?
Citation: Kittawornrat, A., Prickett, J., Chittick, W., Wang, C., Engle, M., Johnson, J., Patnayak, D., Schwartz, T., Whitney, D., Olsen, C., Schwartz, K., Zimmerman, J., Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance?, Virus Research (2010), doi:10.1016/j.virusres.2010.07.025
Abstract: The purpose of this study was to determine whether oral fluid samples could be used to monitorindividually-housed adult boars for porcine reproductive and respiratory syndrome virus(PRRSV) infection. In 3 trials, 24 boars were intramuscularly (IM) inoculated with a modified-live PRRSV (MLV) vaccine (Trial 1), a Type 1 PRRSV isolate (Trial 2), or a Type 2 isolate(Trial 3). Oral fluid samples were collected daily and serum samples were collected twiceweekly. Following the completion of the study, samples were randomized and blind-tested forPRRSV by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR).PRRSV was detected in oral fluids at DPI 1 and all oral fluid specimens were PRRSV qRT-PCRpositive at DPI 4. Although PRRSV was detected in both serum and oral fluid specimensthrough DPI 21, a comparison of matched samples from individual boars showed that oral fluidwas equal to serum for the detection of PRRSV at DPI 7 and more likely to be positive thanserum on DPI 14 and 21. Overall, oral fluid was superior to serum for the detection of PRRSVusing PCR over the 21 day observation period in this study. The results of this experimentsuggest that individually-penned oral fluid sampling could be an efficient, cost-effectiveapproach to PRRSV surveillance in boar studs and other swine populations.
Keywords: PRRSV, surveillance, monitor, diagnosis, detection, oral fluid, serum
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