Influence of age, group size, and presence of PRRSv on oral fluids collection

Vet J. 2019 Feb;244:13-15. doi: 10.1016/j.tvjl.2018.12.005. Epub 2018 Dec 7.

Influence of age, group size and the presence of porcine reproductive and respiratory syndrome virus on the collection of oral fluids.

Graage R1, Hennig-Pauka I2, Arbinger H2, Ritzmann M3, Ladinig A2.

Author information

1

Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, A-1220 Vienna, Austria. Electronic address: rgraage@vetclinics.uzh.ch.

2

Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, A-1220 Vienna, Austria.

3

Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, D-85764 Oberschleißheim, Germany.

Abstract

Pen-based oral fluid (OF) samples have been approved for detecting porcine reproductive and respiratory syndrome virus (PRRSV) RNA and antibodies. The aim of the study was to investigate the influence of age, group size and the presence of PRRSV infection on sampling using pen-based OF collected by presenting pigs with a rope to chew. Samples were collected from pigs of 4/5 weeks of age, 7/8 weeks of age and 12/14 weeks of age. Subsequent PRRSV-PCR and ELISA analyses of OF and serum samples were performed. In this study, 76.5-81.9% of pigs chewed the rope. The group size had significant impact on the pig-rope-interaction (P=0.047). OF and serum had an almost perfect and significant agreement for the detection of antibodies (κ=0.829, P<0.001) and a fair and not significant agreement (κ=0.347, P=0.75) for the detection PRRSV-genome.

Copyright © 2018 Elsevier Ltd. All rights reserved.

KEYWORDS: 

ELISA; Oral fluid; PCR; PRRSV; Rope; Surveillance

PMID:

 

30825888

 

DOI:

 

10.1016/j.tvjl.2018.12.005