In vitro inactivation of PRRSv and PRv by acidic electrolyzed water

Abstract

Slightly acidic electrolyzed water (SAEW, pH 5.0-6.5) is a novel disinfectant with environmentally friendly broad spectrum microbial decontamination properties which could have significant utility on farm. Two of the most important pathogenic viruses in pigs are porcine reproductive and respiratory syndrome virus (PRRSV) and pseudorabies virus (PRV). The aim of this study was to evaluate the viricidal effectiveness of SAEW against PRRSV and PRV in vitro under different available chlorine concentrations (ACCs, 30, 50 and 70mg/L), treatment times (5, 10 and 15min) and temperatures (4, 20, 40 and 60°C), respectively. SAEW had a strong viricidal activity against both PRRSV and PRV. This activity increased with increasing ACC, treatment time and temperature. PRRSV and PRV titres of 7.0log₁₀TCID₅₀/mL and 5.9log₁₀TCID₅₀/mL, respectively, were completely inactivated by SAEW at an ACC of ⩾50mg/L for 10min even though SAEW had no negative effect on the host cells. SAEW thus shows promise as a disinfectant for use on pig farms to reduce the spread of both PRRSV and PRV, and to limit the morbidity associated with those viruses.

Copyright © 2013 Elsevier Ltd. All rights reserved.

Hao X, Shen Z, Wang J, Zhang Q, Li B, Wang C, Cao W. In vitro inactivation of porcine reproductive and respiratory syndrome virus and pseudorabies virus by slightly acidic electrolyzed water. The veterinary journal 2013 [in press].