Kuiek AM, Ooi PT, Yong CK, Ng CF. Comparison
of serum and oral fluid antibody responses after vaccination with a modified
live (MLV) porcine reproductive and respiratory syndrome virus (PPRSV) vaccine
in PRRS endemic farms. Trop Anim Health Prod. 2015 Jun 13. [Epub
ahead of print]
Abstract
Porcine
reproductive and respiratory syndrome (PRRS) is a disease that is both highly
contagious and of great economic importance in Malaysia. Therefore, reliable
and improved diagnostic methods are needed to facilitate disease surveillance.
This study compared PRRSV antibody responses in oral fluid versus serum samples
following PRRS modified live (MLV) vaccination using commercial antibody ELISA
kits (IDEXX Laboratories, Inc.). The study involved two pig farms located in
Perak and Selangor, Malaysia. Both farms were vaccinated with PRRS MLV
1 month prior to sample collection. Thirty-five animals were used as
subjects in each farm. These 35 animals were divided into 7 different
categories: gilts, young sows, old sows, and four weaner groups. Oral fluid and
serum samples were collected from these animals individually. In addition, pen
oral fluid samples were collected from weaner groups. The oral fluid and serum
samples were tested with IDEXX PRRS Oral Fluid Antibody Test Kit and IDEXX PRRS
X3 Antibody Test Kit, respectively. The results were based on sample to
positive ratio (S/P ratio of the samples). Results revealed a significant and
positive correlation between serum and oral fluid samples for both farm A (p = 0.0001,
r = 0.681) and farm B (p = 0.0001, r = 0.601). In general, oral fluids provided
higher S/P results than serum, but the patterns of response were highly
similar, especially for the sow groups. Thus, the use of oral fluids in endemic
farms is effective and economical, particularly for large herds. In conclusion,
the authors strongly recommend the use of oral fluids for PRRS monitoring in
endemic farms.
PMID: 26070293 [PubMed - as supplied by publisher]
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