Arruda AG, Friendship R, Carpenter J, Hand K, Ojkic D, Poljak Z. Investigation of the Occurrence of Porcine Reproductive and Respiratory
Virus in Swine Herds Participating in an Area Regional Control and Elimination
Project in Ontario, Canada. Transbound Emerg Dis. 2015 Mar 11. doi:
10.1111/tbed.12343. [Epub ahead of print]
Abstract
The main
goal of this study was to investigate the occurrence of porcine reproductive
and respiratory syndrome virus (PRRSV)-specific genotypes in swine sites in
Ontario (Canada) using molecular, spatial and network data from a porcine
reproductive and respiratory syndrome (PRRS) regional control project. For each
site, location, animal movement service provider (truck companies), PRRSV
status and sequencing data of the open reading frame 5 (ORF5) were obtained.
Three-kilometre buffers were created to evaluate neighbourhood characteristics
for each site. Social network analysis was conducted on swine sites and
trucking companies to assemble the network and define network components. Three
different PRRSV genotypes were used as outcomes for statistical analysis based
on the region's phylogenetic tree of the ORF5. Multivariable exact logistic
regression was conducted to investigate the association between being positive
for a specific genotype and two main exposures of interest: (i) having at least
one neighbour within three km also positive for the same genotype outside
the production system and (ii) having at least one positive site for the same
genotype in the same truck network component outside the production system.
Results showed that the importance of area spread and truck network on PRRSV
occurrence differed according to genotype. Additionally, the Ontario PRRS
database appears suitable for conducting regional disease investigations.
Finally, the use of relatively new tools available for network, spatial and
molecular analysis could be useful in investigation, control and prevention of
endemic infectious diseases in animal populations.
© 2015
Blackwell Verlag GmbH.
KEYWORDS:
Porcine reproductive and
respiratory syndrome; disease investigation; molecular epidemiology; network
analysis
PMID:
25766306 [PubMed - as supplied by publisher]
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