Patterns of PRRSv and IAV detection in pig oral fluids

Res Vet Sci. 2016 Dec;109:74-80. doi: 10.1016/j.rvsc.2016.09.014. Epub 2016 Sep 21.

Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus(IAV) in oral fluid of pigs.

Biernacka K1, Karbowiak P2, Wróbel P3, Charęza T4, Czopowicz M5, Balka G6, Goodell C7, Rauh R8, Stadejek T9.

Author information

1

Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Department of Pathology and Veterinary Diagnostics, Nowoursynowska 159c, 02-776 Warsaw, Poland.

2

Vet-Com L.L.C., Jagiellońska 71, 10-237 Olsztyn, Poland.

3

Swine Vet Consulting L.L.C., Bolewskiego 40, 63-700 Krotoszyn, Poland.

4

Poldanor SA, Dworcowa 25, 77-320 Przechlewo, Poland.

5

Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Laboratory of Veterinary Epidemiology and Economics, Nowoursynowska 159c, 02-776 Warsaw, Poland.

6

University of Veterinary Medicine, Department of Pathology, István u. 2, H-1078 Budapest, Hungary.

7

IDEXX Laboratories, Inc., Westbrook, ME, USA.

8

Tetracore Inc, 9901 Belward Campus Drive Suite 300, Rockville, MD 20850, USA.

9

Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Department of Pathology and Veterinary Diagnostics, Nowoursynowska 159c, 02-776 Warsaw, Poland. Electronic address: tomasz_stadejek@sggw.pl.

Abstract

Recently oral fluid has become a novel sample type for pathogen nucleic acid and antibody detection, as it is easy to obtain with non-invasive procedures. The objective of the study was to analyze porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs from three Polish production farms, using Real Time PCR and ELISA testing of oralfluid and serum. Oral fluids were collected every 2weeks, in the same 3-4 pens of pigs aged between 5 and 17weeks. Additionally, blood samples were collected every 4weeks from 4 pigs corresponding to the same pens as oral fluid and tested for the presence of PRRSVnucleic acid (pooled by 4) and antibodies. In farm A no PRRSV circulation was detected and only maternal antibodies were present. In farm B and farm C antibodies to PRRSV in serum and oral fluid were detected in most samples. In farm B PRRSV Type 1 was detected in 80.9% of oral fluid samples and in 58.3% of serum pools, and in farm C in 92.8% of oral fluid samples and 75% serum pools. Striking differences were observed between different pens in PRRSV detection patterns. In farms B and C ORF5 sequence analysis showed the presence of wild type strains which were about 84-85% identical to the modified live vaccine used. In all three farms two waves of IAVshedding with oral fluid were detected, in weaners and fatteners.

Copyright © 2016 Elsevier Ltd. All rights reserved.

KEYWORDS: 

ELISA; IAV; Oral fluid; PRRSV; Real time PCR

PMID:

 

27892877

 

DOI:

 

10.1016/j.rvsc.2016.09.014